Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Circ_0011385 increases cisplatin resistance in breast cancer via miR-615-5p suppression

Yiyi Hu, Xiaojing Ye, Feng Xiang, Lei Chen, Peizhen Chen

Department of Pharmacy,The Second Affiliated Hospital of Wenzhou Medical University,Wenzhou City, Zhejiang, China;

For correspondence:- Peizhen Chen Email: ysnvcheasgwo@163.com Tel:+8613566260191

Accepted: 12 January 2024 Published: 29 February 2024

Citation: Hu Y, Ye X, Xiang F, Chen L, Chen P. Circ_0011385 increases cisplatin resistance in breast cancer via miR-615-5p suppression. Trop J Pharm Res 2024; 23(2):243-249 doi: 10.4314/tjpr.v23i2.2

© 2024 The authors.
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Abstract

Purpose: Circular RNAs (circRNAs) participate in chemo-resistance among different cancers, containing breast cancer (BC). Herein, this project aimed to investigate the role of circ_0011385 on cisplatin (DDP) resistance in BC cells.

Methods: circ_0011385 and microRNA (miR)-615-5p content in DDP-resistant BC cells and their parent cells (MCF-7) was calculated by RT-qPCR. CCK-8 assessed inhibition rate, and further to calculate half inhibitory concentration (IC₅₀). Clone formation and flow cytometry experiments detected clone numbers and apoptosis rate. Dual-luciferase reporter system validated targeting between circ_0011385 and miR-615-5p.

Results: circ_0011385 was upregulated, whereas miR-615-5p was decreased in MCF-7/DDP cells. Silencing circ_0011385 or miR-615-5p overexpression enhanced BC cell sensitivity to DDP, repress proliferation, and expedite apoptosis rate. Circ_0011385 directly targeted miR-615-5p.

CONCLUSION: Silencing circ_0011385 increases DDP sensitivity by targeting and up-regulating miR-615-5p expression in MCF-7/DDP.

Keywords: Breast cancer; circ_0011385; cisplatin resistance; miR-615-5p